Review



33342 pi double stain kit  (Beijing Solarbio Science)


Bioz Verified Symbol Beijing Solarbio Science is a verified supplier
Bioz Manufacturer Symbol Beijing Solarbio Science manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Beijing Solarbio Science 33342 pi double stain kit
    33342 Pi Double Stain Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+stain+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pmc12805112-213-8-12
    Average 96 stars, based on 329 article reviews
    33342 pi double stain kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    H&E Stain:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    Staining:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing
    Article Snippet: .. The cells were subsequently stained with the Hoechst 33342/PI Double Stain Kit (Solarbio, Cat# CA1120) for 15 min and imaged using an inverted fluorescence microscope (IX-71, Olympus). ..

    Article Title: NIR Light-Driven Photocatalytic Antibacterial Hydrogels for Synergistic MRSA Biofilm Eradication and Wound Regeneration.
    Article Snippet: .. The cells were subsequently stained using a Hoechst 33342/PI Double Stain Kit (Solarbio, CatCA1120) for 15 min and imaged using an inverted fluorescence microscope. .. HUVECs were cultured in 24-well plates (NEST Biotechnology) at a density of 105 cells ⋅well− 1 and allowed to form a monolayer.

    Article Title: EGB761 ameliorates mild cognitive impairment by inhibiting the pyroptosis and apoptosis in both in vivo and in vitro experiments.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 82174470; Tianjin Natural Science Foundation, Grant/Award Number: 21JCQNJC01170 Abstract Mild cognitive impairment (MCI) is a neurodegenerative condition that is clinically prevalent among the elderly.. EGB761 is widely recognized for its promising therapeutic properties in both the prevention and treatment of neurodegenerative disorders.. The aim of this study was to investigate the effects of EGB761 in MCI and the underlying molecular mechanism.

    Article Title: Lappaconitine hydrobromide prevents NLRP3-GSDMD-mediated pyroptosis to alleviate sepsis-induced acute lung injury by enhancing NR1H3
    Article Snippet: .. 255 256 2.18 Hoechst 33342/PI Staining 257 The cell death rate of THP-1 cells was measured using Hoechst 33342/PI Double Stain Kit (Solarbio, 258 Beijing, China). ..

    Article Title: PRR15 suppresses renal cell carcinoma progression via the NF-κB/FDX1 axis to induce cuproptosis and mitochondrial dysfunction.
    Article Snippet: Cuproptosis is involved in the proliferation, metastasis, and drug resistance formation development of renal cell carcinoma (RCC) by regulating lipid metabolism and oxidative stress levels in the tumor microenvironment, with Ferredoxin 1 (FDX1) as a core regulator.. Proline-rich 15 (PRR15) is a proline-rich protein, that we previously found to inhibit the malignant progression of triplenegative breast cancer through the regulation of the phosphatidylinositol 3-kinase (PI3K) pathway and epithelial-mesenchymal transition (EMT) pathway.. However, the role of PRR15 in cuproptosis and its molecular mechanisms remain unknown.

    Article Title: Inhibition of macrophage pyroptosis protects against sepsis-induced injury to the alveolar epithelial barrier
    Article Snippet: .. Subsequently, the cells were stained using a Hoechst 33342/PI Double Stain Kit (CA1120, Solarbio, Beijing, China) according to the manufacturer’s protocol. .. Briefly, cells were washed with PBS and incubated with Hoechst 33342 (5 μg/mL) and propidium iodide (PI, 5 μg/mL) in the dark at 4 °C for 30 min. After washing with PBS, the stained cells were imaged using a high-content screening (HCS) system (PerkinElmer, Shelton, CT, USA).

    Article Title: NIR Light‐Driven Photocatalytic Antibacterial Hydrogels for Synergistic MRSA Biofilm Eradication and Wound Regeneration
    Article Snippet: .. The cells were subsequently stained using a Hoechst 33342/PI Double Stain Kit (Solarbio, CatCA1120) for 15 min and imaged using an inverted fluorescence microscope. .. HUVECs were cultured in 24‐well plates (NEST Biotechnology) at a density of 10 5 cells·well −1 and allowed to form a monolayer.

    Membrane:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    SDS Page:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    In Situ:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    CCK-8 Assay:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    Recombinant:

    Article Title: NEFL Modulates NRN1-Mediated Mitochondrial Pathway to Promote Diacetylmorphine-Induced Neuronal Apoptosis
    Article Snippet: .. Hematoxylin–eosin (HE) stain kit (G1120, Beijing Solarbio Science & Technology Co., Ltd.), Hoechst 33342/PI Double Stain Kit (CA1120, Beijing Solarbio Science & Technology Co., Ltd.), Reactive Oxygen Species Assay Kit (CA1410, Beijing Solarbio Science & Technology Co.,Ltd), Mitochondrial Membrane Potential Assay Kit with JC-1 (M8650, Beijing Solarbio Science & Technology Co., Ltd), BCA Protein Assay Kit (PC0020, Beijing Solarbio Science & Technology Co., Ltd.), SDS-PAGE Gel Kit (P1200, Beijing Solarbio Science & Technology Co., Ltd.), Nissl staining solutio (G1036, servicebio), In Situ Cell Death Detection Kit, TMR red (12156792910, Roche), CCK-8 (PF0004, Proteintech Group, Inc.), recombinant proteinase K (G1234, servicebio) PE Annexin V Apoptosis Detection Kit I (559763, Becton Dickinson and Company), ultra-ultrasensitive ECL chemiluminescent substrate (BL520A, Biosharp). ..

    Fluorescence:

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing
    Article Snippet: .. The cells were subsequently stained with the Hoechst 33342/PI Double Stain Kit (Solarbio, Cat# CA1120) for 15 min and imaged using an inverted fluorescence microscope (IX-71, Olympus). ..

    Article Title: NIR Light-Driven Photocatalytic Antibacterial Hydrogels for Synergistic MRSA Biofilm Eradication and Wound Regeneration.
    Article Snippet: .. The cells were subsequently stained using a Hoechst 33342/PI Double Stain Kit (Solarbio, CatCA1120) for 15 min and imaged using an inverted fluorescence microscope. .. HUVECs were cultured in 24-well plates (NEST Biotechnology) at a density of 105 cells ⋅well− 1 and allowed to form a monolayer.

    Article Title: EGB761 ameliorates mild cognitive impairment by inhibiting the pyroptosis and apoptosis in both in vivo and in vitro experiments.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 82174470; Tianjin Natural Science Foundation, Grant/Award Number: 21JCQNJC01170 Abstract Mild cognitive impairment (MCI) is a neurodegenerative condition that is clinically prevalent among the elderly.. EGB761 is widely recognized for its promising therapeutic properties in both the prevention and treatment of neurodegenerative disorders.. The aim of this study was to investigate the effects of EGB761 in MCI and the underlying molecular mechanism.

    Article Title: NIR Light‐Driven Photocatalytic Antibacterial Hydrogels for Synergistic MRSA Biofilm Eradication and Wound Regeneration
    Article Snippet: .. The cells were subsequently stained using a Hoechst 33342/PI Double Stain Kit (Solarbio, CatCA1120) for 15 min and imaged using an inverted fluorescence microscope. .. HUVECs were cultured in 24‐well plates (NEST Biotechnology) at a density of 10 5 cells·well −1 and allowed to form a monolayer.

    Microscopy:

    Article Title: Near-infrared light-driven photocatalytic reactive oxygen species-generating antibacterial and self-shrinking hybrid hydrogels for combating drug-resistant bacterial biofilm infection and accelerating wound healing
    Article Snippet: .. The cells were subsequently stained with the Hoechst 33342/PI Double Stain Kit (Solarbio, Cat# CA1120) for 15 min and imaged using an inverted fluorescence microscope (IX-71, Olympus). ..

    Article Title: NIR Light-Driven Photocatalytic Antibacterial Hydrogels for Synergistic MRSA Biofilm Eradication and Wound Regeneration.
    Article Snippet: .. The cells were subsequently stained using a Hoechst 33342/PI Double Stain Kit (Solarbio, CatCA1120) for 15 min and imaged using an inverted fluorescence microscope. .. HUVECs were cultured in 24-well plates (NEST Biotechnology) at a density of 105 cells ⋅well− 1 and allowed to form a monolayer.

    Article Title: EGB761 ameliorates mild cognitive impairment by inhibiting the pyroptosis and apoptosis in both in vivo and in vitro experiments.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 82174470; Tianjin Natural Science Foundation, Grant/Award Number: 21JCQNJC01170 Abstract Mild cognitive impairment (MCI) is a neurodegenerative condition that is clinically prevalent among the elderly.. EGB761 is widely recognized for its promising therapeutic properties in both the prevention and treatment of neurodegenerative disorders.. The aim of this study was to investigate the effects of EGB761 in MCI and the underlying molecular mechanism.

    Article Title: NIR Light‐Driven Photocatalytic Antibacterial Hydrogels for Synergistic MRSA Biofilm Eradication and Wound Regeneration
    Article Snippet: .. The cells were subsequently stained using a Hoechst 33342/PI Double Stain Kit (Solarbio, CatCA1120) for 15 min and imaged using an inverted fluorescence microscope. .. HUVECs were cultured in 24‐well plates (NEST Biotechnology) at a density of 10 5 cells·well −1 and allowed to form a monolayer.



    Similar Products

    96
    Beijing Solarbio Science 33342 pi double stain kit
    33342 Pi Double Stain Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+stain+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pmc12805112-213-8-12
    Average 96 stars, based on 1 article reviews
    33342 pi double stain kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Beijing Solarbio Science hoechst 33342 pi doubles stain kit
    Hoechst 33342 Pi Doubles Stain Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+stain+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pmc13019507-48-0-6
    Average 96 stars, based on 1 article reviews
    hoechst 33342 pi doubles stain kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Beijing Solarbio Science 33342 pi double fluorescence staining kit
    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) <t>Hoechst</t> <t>33342/PI</t> staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
    33342 Pi Double Fluorescence Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+stain+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pmc12930366-124-2-10
    Average 96 stars, based on 1 article reviews
    33342 pi double fluorescence staining kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Beijing Solarbio Science 33342 propidium iodide pi double staining kit
    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) <t>Hoechst</t> <t>33342/PI</t> staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
    33342 Propidium Iodide Pi Double Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+stain+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pm41751830-380-27-32
    Average 96 stars, based on 1 article reviews
    33342 propidium iodide pi double staining kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Beijing Solarbio Science pi dual staining kit
    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) <t>Hoechst</t> <t>33342/PI</t> staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
    Pi Dual Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+stain+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pm41781992-259-11-35
    Average 96 stars, based on 1 article reviews
    pi dual staining kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Beijing Solarbio Science pi double staining kit
    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) <t>Hoechst</t> <t>33342/PI</t> staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
    Pi Double Staining Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+stain+kit/Hoechst+33342%2FPI+Double+Stain+Kit/pmc13029627-195-10-17
    Average 96 stars, based on 1 article reviews
    pi double staining kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) Hoechst 33342/PI staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.

    Journal: Molecular Medicine Reports

    Article Title: Puerarin attenuates myocardial ischemia-reperfusion injury by inhibiting myocardium pyroptosis via the NRF2/HO-1 signaling pathway

    doi: 10.3892/mmr.2026.13824

    Figure Lengend Snippet: Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) Hoechst 33342/PI staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.

    Article Snippet: The Hoechst 33342/PI double fluorescence staining kit (cat. no. CA1120; Beijing Solarbio Science & Technology Co., Ltd.) was used to detect pyroptosis in H9C2 cardiomyocytes from each group.

    Techniques: Staining, Western Blot, Expressing, Over Expression, Negative Control, Binding Assay